ObjectiveTo study the possbility of using intranasal instillation of fine particulate matter (PM2.5) combined with inhalation of ozone (O3) to establish mouse model of combined pulmonary fibrosis and emphysema (CPFE), and to provide a reference for the establishment of CPFE model.MethodsMale C57/BL6 mice were divided randomly into phosphate buffer saline (PBS) intranasal instillation+air inhalation group, PBS intranasal instillation+O3 inhalation group and PM2.5 intranasal instillation+O3 inhalation group, with 8 mice in each group. The mice were intranasally instilled with PBS or PM2.5 suspension (7.8 mg/kg) followed by air or ozone inhalation 24 hours later, twice a week over 8 weeks. Lung function, bronchoalveolar lavage fluid (BALF) cell counts and classification were detected, the pathological changes of lung tissues in hematoxylin-eosin staining were observed, including inflammation scores and mean linear intercept (Lm). The thickness of collagen deposition in subepithelium was measured in lung tissues in Masson staining, and simultaneously hydroxyproline contents in lung tissues were determined.ResultsCompared to PBS instillation+air inhalation group, inspiratory capacity (IC), total lung capacity (TLC) and chord compliance (Cchord) were increased, FEV25 (the forced expiratory volume at 25 ms)/FVC (forced vital capacity) was decreased, total cell counts in BALF, Lm and lung inflammatory scores were increased, the thickness of the subepithelial collagen layer (SEc/Pbm) or hydroxyproline contents was not changed in PBS instillation +O3 inhalation group; IC was decreased, functional residual capacity (FRC) was increased, TLC was increased, Cchord was decreased, FEV25/FVC and FEV50 (the forced expiratory volume at 50 ms)/FVC were decreased, total cell counts in BALF, Lm, lung inflammatory scores, SEc/Pbm and hydroxyproline contents were increased in PM2.5 instillation+O3 inhalation group. Compared to PBS instillation+O3 inhalation group, IC was decreased, FRC was increased, Cchord was decreased, FEV25/FVC and FEV50/FVC were decreased, total cell counts in BALF, Lm, lung inflammatory scores, SEc/Pbm and hydroxyproline contents were increased in PM2.5 instillation +O3 inhalation group.ConclusionCPFE mouse model can be successfully established by PM2.5 intranasal instillation combined with ozone inhalation for consecutive 8 weeks.
ObjectiveTo systematically review the efficacy of different positions on the clinical outcomes of percutaneous nephrolithotomy (PCNL). MethodsThe PubMed, Web of Science, EBSCOhost, Cochrane Library, Embase, CNKI, WanFang Data, VIP, and CBM databases were electronically searched to collect randomized controlled trials (RCTs) related to the objects from January 2014 to April 2024. Two reviewers independently screened literature, extracted data and assessed the risk of bias of the included studies, a network meta-analysis was conducted using Stata 18.0 software. ResultsA total of 54 RCTs involving 5 092 patients and 7 different positions were included. The results of the best probability ranking showed that for positioning time, the split-leg prone position required the shortest time, while the traditional prone position required the longest. For access establishment time, the recumbent lithotomy position had the shortest duration, and the traditional prone position the longest. Intraoperative blood loss was lowest in the supine position and highest in the traditional prone position. Surgical duration was shortest for the recumbent lithotomy position and longest for the traditional prone position. Postoperative hospital stay was shortest for the supine position and longest for the traditional prone position. Complication rates were lowest for the oblique supine position and highest for the traditional prone position. Stone clearance rates were highest for the modified curved prone position and lowest for the traditional prone position. Conclusion Current evidence shows that different body positions have different clinical effects on percutaneous nephrolithotomy. The recumbent lithotomy position may optimize access establishment and surgical duration, while the supine position offers advantages in reducing intraoperative blood loss and shortening postoperative hospitalization. The split-leg prone position minimizes positioning time, the oblique supine position lowers complication rates, and the modified curved prone position maximizes stone clearance. Due to the limited quality and quantity of the included studies, more high quality studies are needed to verify the above conclusion.