THE EXPRESSION OF CELL CYCLE-REGULATED PROTEINS IN PANCREATIC ADENOCARCINOMA AND ITS SIGNIFICANCE_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of General Surgery, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

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Pancreatic neoplasm Cell cycle-regulated protein Immunohistochemistry

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Objective To know the abnormal expression of the cell cycle-regulated proteins in pancreatic adenocarcinoma and their effect on tumor cell growth.
Methods The expression of p16, p21, Rb and p53 protein in 47 cases were investigated by immunohistochemistry with wet autoclave pretreatment for antigen retriaval. Furthermore, tumor growth index were assessed by a novel anti-ki-67 antibody (ki-s5).
Results All the expression of p53, p16, p21 and Rb protein were the nuclear stainning. The positive rates of p53, p16, p21 and Rb protein were 55%, 53%, 74% and 98% respectively. There was negative correlation between of p16, p21 or Rb protein expression and ki-67 growth index. No relation of p53 protein stainning and the expression of p21 protein was found.
Conclusion In pancreatic adenocarcinoma, the negative expression of p16 protein and p21 protein may play an important role in tumor cell growth, but tumor proliferation caused by abnormality of Rb protein is rare. The expression of p21 protein was not associated with the expression of p53 protein.

Citation： WANG Zhong,YAN Lnan,LI Li,et al.. THE EXPRESSION OF CELL CYCLE-REGULATED PROTEINS IN PANCREATIC ADENOCARCINOMA AND ITS SIGNIFICANCE. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(5): 279-281. doi: Copy

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1. Dei Tos AP, Maestro R, Doglioni C, et al. Tumour suppressor genes and related molecules in leiomyosarcoma 〔Ｊ〕. Am J Pathol, 1996; 148(4)∶1037.
2. Kloppel G, Mailet B. Classification and staging of pancreatic nonendocrine tumours 〔Ｊ〕. Radiologic Clinics of North America, 1989; 27(1)∶105.
3. Geradts J,Kratzke RA, Niehans GA, et al. Immunohistochemical detection of the cyclindependent kinase inhibitor 2/multiple tumour suppressor gene 1 (CDKN2/MTS1) product p16ink4 in archival human solid tumours: correlation with retinoblastoma protein expression 〔Ｊ〕. Cancer Res, 1995; 55(24)∶6006.
4. Bankfalvi A, Navabi H, Bier B, et al. Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry 〔Ｊ〕. J Pathol, 1994; 174(3)∶223.
5. Quinn CM, Wright NA. The clinical assessment of proliferation and growth in human tumours: evaluation of methods and applications as prognostic variables 〔Ｊ〕. J Pathol, 1990; 160(2)∶93.
6. Kreipe H, Wacker HH, Heidebrecht HJ, et al. Determination of the growth fraction in nonhodgkin, s lymphoma by monoclonal antibody kis5 directed against a formalinresistent epitope of the ki67 antigen 〔Ｊ〕. Am J Pathol, 1993; 142(6)∶1689.
7. Digiuseppe D, Redston MS, Yeo CJ, et al. p53independent expression of cyclindependent kinase inhibitor p21 in pancreatic carcinoma 〔Ｊ〕. Am J Pathol, 1995; 147(4)∶884.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

THE EXPRESSION OF CELL CYCLE-REGULATED PROTEINS IN PANCREATIC ADENOCARCINOMA AND ITS SIGNIFICANCE

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