EXPERIMENTAL STUDY ON THE TOLERANCE OF NORMOTHERMIC HEPATIC INFLOW OCCLUSION WITH PORTAL BLOOD BYPASS IN RAT_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038;

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Rat Liver Ischemia-reperfusion injury

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Objective To investigate the maximum tolerance limit of rats to hepatic inflow occlusion with portal vein blood bypss (PBB) in normothermia.
Methods First. A new animal model was established, the animal survival rate were calculated following 7 days of reperfusion after hepatic inflow occlusion of 30, 60, 90, 100, 110, 120 min or portal triad clamping (PTC) of 30 min. And then, the hepatic energy metabolism (RCR, P/O, ATP, AKBR) was studied following 30, 90, 120 min of ischemia or 1, 6, and 24 hours of reperfusion after the ischemia. According to the reversibility of the hepatic motochondrial function injury and maximum as long as a period of liver warm ischemia of all animal postoperative 7 days survial, the safe limit of rat to hepatic inflow occlusion was evaluated.
Results The survival rate on postoperative 7 days was one hundred percent subjected to 30, 60 and 90 min of hepatic inflow occlusion, and 50, 30, 20 percent in 100, 110, 120 min, respectively, the survival rate in rats with 30 min of portal triad champing was about 40 percent. The parameters of hepatic motochondrial function reflecting the degree of liver damage to ischemia showed significantly different as compared to sham group. The functional lesion was exacerbated during inital reperfusion, then was restored progressively in PBB-30 min and PBB-90 min groups, but was maintained low level in PBB-120 min and PTC-30 min groups.
Conclusion The 90 minutes is the maximum limit of rats to hepatic inflow occlusion in normothermia.

Citation： HE Xiaodong,DONG Jiahong,CAI Jingxiu,et al.. EXPERIMENTAL STUDY ON THE TOLERANCE OF NORMOTHERMIC HEPATIC INFLOW OCCLUSION WITH PORTAL BLOOD BYPASS IN RAT. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(4): 220-223. doi: Copy

1.	Nuzzo G, Giuliante F, Giovannini I, et al. Hepatic resections in normothermic ischemia 〔Ｊ〕. Surgery, 1996; 120(5)∶825.
2.	何效东，董家鸿，蔡景修等. 门静脉转流下入肝血流阻断动物模型的建立及评价〔Ｊ〕. 西北国防医学杂志， 1999； 20(3)∶175.
3.	Johnson D. Isolation of liver or kidney mitochondria 〔Ｊ〕. Methods in Enzymol, 1967; 10(1)∶94.
4.	Lowry OH. Protein measurement with the Follinphenal legent 〔Ｊ〕. J Biol Chem, 1951; 193(2)∶265.
5.	Estabrood RW. Mitochondrial respiratory control and the polarographic measurement of ADP/O ratio 〔Ｊ〕. Methods In Enzymol, 1967; 10(1)∶41.
6.	严涛，张平，段恒春等. 组织ATP、ADP、AMP水平的荧光素酶系测定〔Ｊ〕. 第三军医大学学报， 1987； 9(3)∶294.
7.	Li PK, Lee JT, MacGillivray MH, et al. Direct, fixedtime kinetic assays for βhydrosy butyrate and acetoacetate with a centrifugac analyzer or a computerbacked spectrophotometer 〔Ｊ〕. Clin Chem, 1980; 26(12)∶1713.
8.	Isozaki H, Okajima K, Kobayashi M, et al. Experimental study of liver injury after partial hepatectomy with intermittent or continuous hepatic vascular occlusion 〔Ｊ〕. Eur Surg Res, 1995; 27(2)∶313.
9.	GonzalezElecha B, Cutrin JC, Boveris A. Time course and mechanism of oxidative stress and tissue damage in rat liver subjected to in vivo ischemiareperfusion 〔Ｊ〕. J Clin Invest, 1993; 91(2)∶456.
10.	Ozawa K, Aoyama H, Yasuda K, et al. Metabolic abnormalities associated with postoperative organ failure: a redox theory 〔Ｊ〕. Arch Surg, 1983; 118(11)∶1245.
11.	Delva E, Barberousse JP, Nordlinger B, et al. Hemodynamic and biochemical monitoring during major liver resection with use of hepatic vascular exclusion 〔Ｊ〕. Surgery, 1984; 95(3)∶309.
12.	Ferri M, Gabriel S, Gavelli A, et al. Bacterial translocation during portal clamping for liver resection: a clinical study 〔Ｊ〕. Arch Surg, 1997; 132(FEB)∶162.

1. Nuzzo G, Giuliante F, Giovannini I, et al. Hepatic resections in normothermic ischemia 〔Ｊ〕. Surgery, 1996; 120(5)∶825.
2. 何效东，董家鸿，蔡景修等. 门静脉转流下入肝血流阻断动物模型的建立及评价〔Ｊ〕. 西北国防医学杂志， 1999； 20(3)∶175.
3. Johnson D. Isolation of liver or kidney mitochondria 〔Ｊ〕. Methods in Enzymol, 1967; 10(1)∶94.
4. Lowry OH. Protein measurement with the Follinphenal legent 〔Ｊ〕. J Biol Chem, 1951; 193(2)∶265.
5. Estabrood RW. Mitochondrial respiratory control and the polarographic measurement of ADP/O ratio 〔Ｊ〕. Methods In Enzymol, 1967; 10(1)∶41.
6. 严涛，张平，段恒春等. 组织ATP、ADP、AMP水平的荧光素酶系测定〔Ｊ〕. 第三军医大学学报， 1987； 9(3)∶294.
7. Li PK, Lee JT, MacGillivray MH, et al. Direct, fixedtime kinetic assays for βhydrosy butyrate and acetoacetate with a centrifugac analyzer or a computerbacked spectrophotometer 〔Ｊ〕. Clin Chem, 1980; 26(12)∶1713.
8. Isozaki H, Okajima K, Kobayashi M, et al. Experimental study of liver injury after partial hepatectomy with intermittent or continuous hepatic vascular occlusion 〔Ｊ〕. Eur Surg Res, 1995; 27(2)∶313.
9. GonzalezElecha B, Cutrin JC, Boveris A. Time course and mechanism of oxidative stress and tissue damage in rat liver subjected to in vivo ischemiareperfusion 〔Ｊ〕. J Clin Invest, 1993; 91(2)∶456.
10. Ozawa K, Aoyama H, Yasuda K, et al. Metabolic abnormalities associated with postoperative organ failure: a redox theory 〔Ｊ〕. Arch Surg, 1983; 118(11)∶1245.
11. Delva E, Barberousse JP, Nordlinger B, et al. Hemodynamic and biochemical monitoring during major liver resection with use of hepatic vascular exclusion 〔Ｊ〕. Surgery, 1984; 95(3)∶309.
12. Ferri M, Gabriel S, Gavelli A, et al. Bacterial translocation during portal clamping for liver resection: a clinical study 〔Ｊ〕. Arch Surg, 1997; 132(FEB)∶162.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

EXPERIMENTAL STUDY ON THE TOLERANCE OF NORMOTHERMIC HEPATIC INFLOW OCCLUSION WITH PORTAL BLOOD BYPASS IN RAT

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