Objective
To investigate
the effects of inhaled prostaglandin E1 （PGE1）on Th1/Th2 polarity in rat model of lipopolysaccharide（LPS） induced acute lung injury（ALI）.Methods Healthy adult male Wistar rats [weight （200±20）g] were randomly divided into normal control（NS） group，LPS group and PGE1 group.The model of ALI were established by injecting LPS of 5 mg/kg into caudal vein.The rats in PGE1 group inhaled aerosolized PGE1（2 μg/mL）for 30 minutes after LPS injection,then repeat the procedure 12 hours later. 1 h，6 h，12 h and 24 h after last PGE1 inhalation,enzyme linked immunosorbant assay （ELISA） was empolyed to measure the level of interferon-γ（IFN-γ）and interleukin-4（IL-4）in the serum and bronchoalveolar lavage fluid（BALF）and the ratio of IFN-γ/IL-4（Th1/Th2）was calculated.Pathological examination was made under light microscope.Results Pathological examination of lung tissue demonstrated success ALI model.Compared to NS group,the ratio of IFN-γ/IL-4（Th1/Th2）both in serum and BALF in LPS group elevated significantly（P lt;0.01）. PGE1 administration significantly decreased the ratio IFN-γ/IL-4 in serum after 6h（P lt;0.01）and in BALF at all time points（P lt;0.01）.Conclusion The imbalance of was found in the LPS induced ALI，inhaled PGE1 aerosol inhalation could restore Th1/Th2 cytokine balance in the rats model induced by LPS.
Citation：
FU Mingsheng ,XIAO Xiaohui,WAN Yixin.. The effects of inhaled prostaglandin E1 on Th1/Th2 lipopolysaccharideinduced acute lung injury in rats. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(1): 47-51. doi:
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Copyright © the editorial department of Chinese Journal of Respiratory and Critical Care Medicine of West China Medical Publisher. All rights reserved
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De perrot M，Fischer S，Liu M，et al.Prostaglandin E1 protects lung 30 transplants from ischemia-reperfusion injury：a shift from pro to anti inflammatory cytokines.Transplantation，2001，72：1505-1512.
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Chopra J，Webster RO.PGE1 inhibits neutrophil adherence and neutrophil-mediared injury to cultured endothelial cells.Am Rev Respir Dis，1998，138：915.
|
| 11. |
Tamura DY，Moore EE，Partrick DA，et al.Prostaglandin E1 attenuates cytotoxic mechanisms of primed neutrophils.Shock，1998，9：171-176.
|
| 12. |
Bao S，Wang Y，Sweeney P，et al.Keratinocyte growth factor induces Akt kinase activity and inhibits Fas-mediated apoptosis in A549 lung epithelial cells.Am J Physiol Lung Cell Mol Physiol，2005，288：L36-L42.
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| 13. |
岳子勇，宋春雨，李文志，等.雾化吸入PGE1对急性肺损伤猪氧合及肺内分流的影响.中华麻醉医学杂志，2006，26：435-438. 14 Schmid -Weber CB，Alexander SI，Henault L E，et al.IL–4 enhances IL-10 gene expression inmurine TH2 cells in the absence of TCR engagement [J].Immunol，1999，162：238.李洪霞，张进川，赵亚力等，地塞米松对急性肺损伤大鼠炎症介质/抗炎介质表达的影响.中国呼吸与危重监护杂志.2005，4(1)：65-68.
|
- 1. 1
Tamura DY
， Moore EE ，Partrick DA，et al.Prostaglandin E1 attenuates cytotoxic mechanisms of primed neutrophils.Shock，1998，9：171-176.
- 2. Zedler S，Bone RC，Baue AE，et al.T-cell reactivity and its predictive role in immuno- suppression after burns.Crit Care Med，1999，27：66-72.
- 3. 冯英凯，徐剑铖，杨庆华，等.内毒素肺损伤大鼠Th1、Th2细胞凋亡的变化及其作用探讨.重庆医学，2005，34：74-77.
- 4. 韦俊，梁自乾，孙秀锋.早期雾化吸入前列腺素E1对大鼠烟雾吸入性肺损伤的保护作用.广西医科大学学报，2006，23：266-269.
- 5. Marsh CB，Wewer MD.The pathogenesis of sepsis：factor that modulate the response to gram-negative bacterial infection.Clin Chest Med，1996，17：183-197.
- 6. Li B，Toumier C，Divis RJ，et al.Regulation of IL-4 expression by the transcription factor Jun B during T helper cell differentiation.EMBO J，1999，18：420-432.
- 7. Traber DL，Linares HA，Herndon DN.The pathophysiology of inhalation injury a review.Burns，1998，14：357.
- 8. Wajima I，Shiga T，Yoshikawa T，et al.Intravenous alprostadil，an analog of prostaglandin E1，prenents thiamyal fentanyl induced bronchoconstriction in humans.Anesth Analg，2003，97：456-460.
- 9. De perrot M，Fischer S，Liu M，et al.Prostaglandin E1 protects lung 30 transplants from ischemia-reperfusion injury：a shift from pro to anti inflammatory cytokines.Transplantation，2001，72：1505-1512.
- 10. Chopra J，Webster RO.PGE1 inhibits neutrophil adherence and neutrophil-mediared injury to cultured endothelial cells.Am Rev Respir Dis，1998，138：915.
- 11. Tamura DY，Moore EE，Partrick DA，et al.Prostaglandin E1 attenuates cytotoxic mechanisms of primed neutrophils.Shock，1998，9：171-176.
- 12. Bao S，Wang Y，Sweeney P，et al.Keratinocyte growth factor induces Akt kinase activity and inhibits Fas-mediated apoptosis in A549 lung epithelial cells.Am J Physiol Lung Cell Mol Physiol，2005，288：L36-L42.
- 13. 岳子勇，宋春雨，李文志，等.雾化吸入PGE1对急性肺损伤猪氧合及肺内分流的影响.中华麻醉医学杂志，2006，26：435-438. 14 Schmid -Weber CB，Alexander SI，Henault L E，et al.IL–4 enhances IL-10 gene expression inmurine TH2 cells in the absence of TCR engagement [J].Immunol，1999，162：238.李洪霞，张进川，赵亚力等，地塞米松对急性肺损伤大鼠炎症介质/抗炎介质表达的影响.中国呼吸与危重监护杂志.2005，4(1)：65-68.
