To investigate the mRNA expression of nm23-H1 gene in human liver tumor. In tumor and corresponding nontumoral liver specimens from 20 patients, nm23-H1 mRNA were examined by reverse transcriptionpolymerase chain reaction (RT-PCR) method with specific primers. Results: The primers designed in this study could amplified nearly entire coding sequence of nm23-H1 gene. All the samples showed positive expression of nm23-H1 mRNA, indicating there was no expression loss or obvious alteration. Conclusions: The achievement of RT-PCR method lays foundation for quantitative gaugement of nm23-H1 mRNA in liver tumor.
Citation：
Xu Xiao,Zheng Shusen,Chen Zhi,et al.. mRNA EXPRESSION OF nm23-H1 GENE IN HUMAN LIVER TUMOR ASSAYED BY REVERSE TRANSCRIPTIONPOLYMERASE CHAIN REACTION. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1999, 6(4): 204-206. doi:
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Wang L, Patel U, Ghosh L, et al. Mutation in the nm23 gene is associated with metastasis in colorectal cancer. Cancer Res, 1993; 53(2)∶717.
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Yamaguchi A, Urano T, Goi T, et al. Expression of human nm23H1 and nm23H2 proteins in hepatocellular carcinoma. Cancer, 1994; 73(9)∶2280.
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|
- 1. Stahl JA, Leone A, Rosengard AM, et al. Identification of a second human nm23 gene, nm23H2. Cancer Res, 1991; 51(1)∶445.
- 2. Okada K, Urano T, Goi T, et al. Isolation of human nm23 genomes and analysis of loss of heterozygosity in primary colorectal carcinomas using a specific genomic probe. Cancer Res, 1994; 54(15)∶3979.
- 3. Takebe Y, Seiki M, Fujisawa J, et al. SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the RU5. Mol Cell Biol, 1988; 8(4)∶466.
- 4. Steeg PS, Bevilacqua G, Kopper L, et al. Evidence for a novel gene associated with low tumor metastatic potential. J Natl Cancer Inst, 1988; 80(3)∶200.
- 5. Caligo MA, Cipollini G, Fiore L, et al. nm23 gene expression correlates with cell growth rate and sphase. Int J Cancer, 1995; 60(6)∶837.
- 6. Steeg PS. Metastasis. Cancer Res, 1995; 36(Suppl)∶650.
- 7. Leone A, McBride OW, Weston A, et al. Somatic allelic deletion of nm23 in human cancer. Cancer Res, 1991; 51(9)∶2490.
- 8. Wang L, Patel U, Ghosh L, et al. Mutation in the nm23 gene is associated with metastasis in colorectal cancer. Cancer Res, 1993; 53(2)∶717.
- 9. Yamaguchi A, Urano T, Goi T, et al. Expression of human nm23H1 and nm23H2 proteins in hepatocellular carcinoma. Cancer, 1994; 73(9)∶2280.
- 10. Boix L, Bruix J, Campo E, et al. nm23H1 expression and disease recurrence after surgical resection of small hepatocellular carcinoma. Gastroenterology, 1994; 107(2)∶486.
