ObjectiveTo investigate the relationship between peripheral T cell apoptosis and specific immune tolerance induced by T cell vaccination（TCV）. MethodsT cell vaccinations were made from the spleen cells of SD rats, which were induced by ConA and were challenged with the spleen cells of Wistar rats. Normal SD rats were vaccinated intraperitoneally with TCV (experimental group) or RPMI 1640 culture buffer (control group) respectively .Oneway mixed lymphocyte reaction (MLR) were performed,the apoptosis of peripheral T cell were assayed using flow cytometric analysis before and after vaccination.ResultsIn experimental group, the result of MLR showed that the response captivity of SD rat spleen cells were suppressed significantly after vaccination in comparison with prevaccination (P lt;0.01) and the percentage of peripheral T cell apoptosis was increased significantly after vaccination compared with prevaccination (P lt;0.01); In control group, there was no significant difference between prevaccination and postvaccination about MLR and peripheral T cell apoptosis. ConclusionT cell vaccination is capable of inducing Agspecific immune tolerance, the T cell apoptosis of peripheral blood induced by T cell vaccination may result in the depletion of Agspecific reactive T cells, which is vital in inducing specific immune tolerance.
Citation：
ZHAO Zhenlin,GUO Yongzhang,LI Li,ZHANG Jie,ZHANG Rui. An Investigation of the Relationship Between Peripheral T Cell Apoptosis and Specific Immune Tolerance Induced by T Cell Vaccination. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2003, 10(4): 359-361. doi:
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- 2. Gudmundsdottir H, Turka LA. Transplantation tolerance: mechanisms and strategies? [Ｊ]. Semin Nephrol， 2000; 20(2)∶209.
- 3. 李天发,屠宏庆.移植免疫耐受、移植排斥与细胞凋亡 [Ｊ]. 国外医学免疫性分册， 2000; 23(3)∶162.
- 4. Hermans G, Denzer U, Lohse A,et al. Cellular and humoral immune responses against autoreactive T cells in multiple sclerosis patients after T cell vaccination [Ｊ]. J Autoimmun, 1999; 13(2)∶233.
- 5. Zang YC, Hong J, Rivera VM, et al. Preferential recognition of TCR hypervarible regions by human antiidiotypic T cells induced by T cell vaccination [Ｊ]. J Immunol, 2000; 164(8)∶4011.
- 6. 陈永良,何球藻. 移植免疫中T细胞疫苗作用机制的初步分析 [Ｊ]. 上海免疫学杂志, 1996; 16（3）∶138.
- 7. Kabelitz D, Pohl T,Pechhold K.T cell apoptosis triggered via the CD3/T cell receptor complex and alternative activation pathways [Ｊ]. Curr Top Microbiol Immunol， 1995; 200(1)∶1.
